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Culturing Nerve Cells, Second Edition
Kimberley Goslin
2ndPublisher: Bradford BookFormat: Hardcover
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. It also provides an understanding of the principles behind the protocols. In effect the contributors invite you to their labs and provide information obtainable from such a visit. The authors of the introductory chapters present the fundamental principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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